Isesengura ryubuhanga bwa peptide

Peptide isa na tekinoroji ya synthesis

Ubushakashatsi niterambere ryimiti ya peptide iratera imbere byihuse mubuvuzi.Nyamara, iterambere ryimiti ya peptide igarukira kubiranga.Kurugero, kubera sensibilité idasanzwe kuri hydrolysis ya enzymatique, ituze riragabanuka, kandi ihinduka ryimiterere ihinduka rituma habaho intego zidasanzwe, hydrophobique nkeya, no kubura uburyo bwihariye bwo gutwara abantu.Kugirango tuneshe izo peptide, twasabye ibisubizo byinshi no gukoresha neza ubwoko bumwe bwa peptide nimwe murimwe.

Ubwoko bwa peptide (izina ry'icyongereza: Peptoid) cyangwa Poly - N - mu mwanya wa glycine (izina ry'icyongereza: Poly real - N - insimburangingo), ni peptide ya peptide ya peptide mumurongo munini.Urunigi rwa alpha karubone rwimura urunigi nyamukuru azote aho kuba urunigi.Muri polypeptide yumwimerere, itsinda R ryuruhererekane rwa aminide acide amine acide 20 zitandukanye, ariko itsinda R rifite amahitamo menshi muri peptoid.Muri peptide, peptide kumurongo wingenzi wa acide amine muri azote ya karubone ya azote aho guhinduranya urunigi kumurongo nyamukuru.Twabibutsa ko peptide muri rusange idatanga urwego rwohejuru rwateganijwe nkurwego rwa kabiri muri peptide na proteyine kubera kubura hydrogène kuri azote yumugongo.Peptide intego yambere ni uguteza imbere na protease peptide yibiyobyabwenge bito.

Isesengura ryubuhanga bwa peptide

Uburyo bwa synthesis ya peptide bwatangijwe

Ubusanzwe uburyo bwa peptide bumeze nka synthesis nuburyo bwa subingle synthesis uburyo bwahimbwe na RonZuckermann, buri kimwekimwe kigabanyijemo intambwe ebyiri: acylation no kwimurwa.Muri acylation, intambwe yambere nugukora aside ya haloacetike kugirango ikore hamwe na amine asigaye kumpera yintambwe ibanziriza iyi, cyane cyane diisopropyl carbone diimine.bromoaceticacid yakoreshwaga na diisopropylcarbodiimide."Mu myitwarire yo gusimbuza (reaction ya bimolecular nucleophilique reaction), amine, ubusanzwe yibanze, yibasira halogen iyindi kugirango ikore glycine yasimbuwe."Inzira ya sintetike ya subunitar ikoresha amine yibanze iboneka byoroshye kubyara peptide, bityo bigafasha synthesis ya peptide.

Kwiyongera gukomeye mubyiciro bya peptide synthesis ifite uburambe bukomeye, irashobora kuguha serivisi zitandukanye za peptide ya synthesis.

Isesengura ryubuhanga bwa peptide

Ibyiza bya peptide

Birahamye cyane: peptoide ihagaze neza muri vivo kuruta peptide.

Guhitamo byinshi: Peptoide ikwiranye nubushakashatsi bwavumbuwe hamwe kubera ko ubwoko bwinshi bwububiko butandukanye bwa polypeptide bushobora kuboneka muguhindura umugongo amino.

Birenzeho: Ubwinshi bwimiterere ya peptoid irashobora gutuma peptoide ihitamo neza uburyo bwo gusikana uburyo bwo kubona vuba ibintu byihariye bihuza na poroteyine.

Ubushobozi bwisoko ryinshi: ibiranga ubwoko bwa peptide reka reka bihinduke ubwoko bwiterambere ryibiyobyabwenge bifite amahirwe menshi.