Ibisobanuro
OVA Peptide (257-264) TFA nicyiciro cya I (Kb) cyagabanijwe peptide epitope ya OVA, peptide octamer ikomoka kuri ovalbumin yatanzwe nicyiciro cya I MHC molekile H-2Kb.TAP1-I- na C57BL / 6 macrophage irashobora gutunganya Crl-OVA hamwe nuburebure bwa OVA mubice bitandukanye bya selile, kandi protein yibanze ya OVA peptide (257-264) epitopes igira ingaruka kuri TAP yigenga itunganijwe ryicyiciro cya MHC I.
Ibisobanuro
Kugaragara: Ifu yera-yera-ifu
Isuku (HPLC):≥98.0%
Umwanda umwe:≤2.0%
Ibirimo Acetate (HPLC): 5.0%~12.0%
Ibirimo Amazi (Karl Fischer):≤10.0%
Peptide Ibirimo:≥80.0%
Gupakira no kohereza: Ubushyuhe buke, gupakira vacuum, neza kuri mg nkuko bisabwa.
Nigute ushobora gutumiza?
1. Contact us directly by phone or email: +86-13735575465, sales1@gotopbio.com.
2. Tegeka kumurongo.Nyamuneka wuzuze urupapuro rwabigenewe.
3. Tanga izina rya peptide, CAS Oya cyangwa urutonde, ubuziranenge no guhindura niba bikenewe, ubwinshi, nibindi tuzatanga cote mumasaha 2.
4. Tegeka guhinduka ukoresheje amasezerano yo kugurisha yasinywe byemewe na NDA (amasezerano yo kutamenyekanisha) cyangwa amasezerano yibanga.
5. Tuzakomeza kuvugurura iterambere ryateganijwe mugihe.
6. Gutanga peptide na DHL, Fedex cyangwa abandi, na HPLC, MS, COA bizatangwa hamwe n'imizigo.
7. Politiki yo gusubizwa izakurikizwa niba hari itandukaniro ryubwiza cyangwa serivisi.
8. Serivisi nyuma yo kugurisha: Niba abakiriya bacu bafite ikibazo kijyanye na peptide mugihe cyibigeragezo, nyamuneka twandikire natwe tuzagusubiza mugihe gito.
Ibicuruzwa byose byikigo bikoreshwa gusa mubushakashatsi bwubumenyi, ni's bibujijwe gukoreshwa mu buryo butaziguye n'abantu bose ku mubiri w'umuntu.
Ibibazo:
Nihe mpanuro nziza kubushakashatsi bwanjye?
Mburabuzi, peptide irangirana na N-terminal yubusa amino hamwe na C-terminal yubusa ya carboxyl.Urutonde rwa peptide akenshi rugereranya urukurikirane rwa poroteyine ya nyina.Kugirango wegere poroteyine ya nyina, iherezo rya peptide rikenera gufungwa, ni ukuvuga n-terminal acetylation na C-terminal amidation.Ihinduka ryirinda kwinjiza amafaranga arenze, kandi rituma rishobora kurushaho gukumira ibikorwa bya exonucliase, kugirango peptide ihamye.
Nibihe byahinduwe byanditseho polypeptide bishobora guhuzwa muri peptide yubushinwa?
Isosiyete yacu itanga ibimenyetso bitandukanye byahinduwe peptide, nka acetylation, label ya biotine, guhindura fosifora, guhindura fluorescence, nabyo birashobora gutegurwa ukurikije ibyo ukeneye bidasanzwe.
Nigute ushobora gushonga polypeptide?
Ubushobozi bwa polypeptide buterwa ahanini nuburyo bwibanze nubwa kabiri, imiterere yo guhindura ikirango, ubwoko bwa solvent hamwe nibitekerezo byanyuma.Niba peptide idashonga mumazi, ultrasound irashobora gufasha kuyishonga.Kuri peptide yibanze, birasabwa gushonga hamwe na 10% acide acetike;Kuri peptide acide, birasabwa guseswa na 10% NH4HCO3.Amashanyarazi kama arashobora kandi kongerwamo polypeptide idashonga.Peptide ishonga muburyo buke bwa solge organic (urugero, DMSO, DMF, inzoga ya isopropyl, methanol, nibindi).Birasabwa cyane ko peptide yashonga mumashanyarazi yabanje hanyuma ikongerwaho buhoro buhoro mumazi cyangwa izindi buffer kugeza igihe byifuzwa.
Ni ubuhe buryo bwiza bwo kubungabunga?Peptide ihagaze gute?
Nyuma ya lyofilique, polypeptide irashobora gukora ifu ya fluff cyangwa flocculant, ishobora kwirinda kwangirika hakiri kare ya polypeptide.Ibyifuzo byububiko bisabwa: a.-20℃kubika cyangwa ibidukikije byumye b.Gerageza kwirinda guhagarika inshuro nyinshi c.Gerageza kwirinda kubika muburyo bwo gukemura (ifu yumye-yumye irashobora kubikwa mubipaki bitandukanye kugirango byoroshye gukoresha) d.Niba igomba kubikwa mubisubizo, birasabwa gushonga peptide mumazi meza mugihe acide nkeya hanyuma ukabika kuri -20℃.
Nigute peptide yanjye itwarwa?Ni izihe raporo y'ibizamini zitangwa?
Polypeptide zose zumye zikonjeshwa mubisanzwe zibikwa mubintu byihariye bya ml 2 cyangwa 10ml hamwe namakuru yambere yisesengura hamwe na raporo ya synthesis ikubiyemo amakuru yingenzi nkurukurikirane, uburemere bwa molekile, ubuziranenge, uburemere, numubare wa polypeptide.
Uburemere bwa net ni iki?Ibirimo peptide ni iki?
Nyuma ya peptide ya lyofilique isanzwe ifite fluff kandi isa na fluff, irashobora kuba irimo amazi menshi, amazi ya adsorbed hamwe numunyu bitewe nibiranga peptide ubwayo.Ibi ntibisobanura ko ubuziranenge bwa peptide budahagije, ariko ko ibirimo peptide bigabanukaho 10% kugeza 30%.Uburemere bwa peptide nuburemere nyabwo bwa peptide ukuyemo amazi na ion protonone.Kugirango habeho kwibumbira hamwe kwa peptide, ibintu bitari peptide bigomba gukurwa kuri peptide ya peteroli.
Peptide ishobora kuba nziza gute?
Isosiyete yacu irashobora gutanga urwego rutandukanye rwabakiriya guhitamo, kuva kubutare kugeza kuri> 99.9%.Ukurikije ibyo abakiriya bakeneye dukeneye gutanga ubuziranenge> 99,9% ultra-pure polypeptide.