Nibikoresho bisobanutse neza, HPLC irashobora kuganisha byoroshye kubibazo bito bito niba bidakozwe muburyo bwiza mugihe cyo gukoresha.Kimwe mubibazo bikunze kugaragara ni ikibazo cyo guhagarika inkingi.Nigute ushobora gukemura vuba chromatografi idakwiye.Sisitemu ya HPLC igizwe ahanini nicupa ryibigega, pompe, inshinge, inkingi, icyumba cyubushyuhe bwinkingi, detector hamwe na sisitemu yo gutunganya amakuru.Kuri sisitemu yose, inkingi, pompe na detector nibyo bice byingenzi nibibanza nyamukuru bikunda ibibazo.
Urufunguzo rwumuvuduko winkingi nigice gikeneye kwitabwaho cyane mugihe ukoresheje HPLC.Ihagarikwa ryumuvuduko winkingi rifitanye isano rya hafi na chromatografi yimiterere yimiterere, inkingi ikora neza, gutandukana neza nigihe cyo kugumana.Umuvuduko winkingi winkingi ntabwo bivuze ko agaciro kumuvuduko uhagaze neza kubiciro bihamye, ahubwo ni uko ihindagurika ryumuvuduko uri hagati ya 345kPa cyangwa 50PSI (kwemerera gukoresha ikoreshwa rya elisiyoneri mugihe umuvuduko winkingi uhagaze kandi uhinduka buhoro).Umuvuduko mwinshi cyangwa muto cyane nikibazo cyingutu.
Bikunze kugaragara kunanirwa kwa HPLC nibisubizo
1, umuvuduko mwinshi nikibazo gikunze gukoreshwa mugukoresha HPLC.Ibi bivuze kuzamuka gitunguranye.Muri rusange, hari impamvu zikurikira: (1) Muri rusange, ibi biterwa numuyoboro utemba.Aha, tugomba kubisuzuma kimwe.a.Ubwa mbere, gabanya inleti ya pompe vacuum.Kuri ubu, umuyoboro wa PEEK wuzuyemo amazi kuburyo umuyoboro wa PEEK wari muto kuruta icupa rya solve kugirango urebe niba amazi yatonyanga uko bishakiye.Niba amazi adatonyanga cyangwa atemba gahoro, umutwe wa filteri yumutwe urahagarikwa.Umuti: Shira muri acide ya nitric 30% mugice cyisaha hanyuma ukarabe namazi ya ultrapure.Niba amazi yatonyanga bidatunguranye, umusemburo wa filteri umutwe ni ibisanzwe kandi urimo kugenzurwa;b.Fungura icyuma cya Purge kugirango icyiciro kigendanwa kitanyuze mu nkingi, kandi niba igitutu kitagabanutse cyane, akayunguruzo umutwe wera urahagaritswe.Umuti: Akayunguruzo kayunguruzo kavanyweho hanyuma kongerwamo 10% isopropanol mugice cyisaha.Dufashe ko umuvuduko ugabanuka munsi ya 100PSI, umutwe wera uyungurujwe ni ibisanzwe kandi urimo kugenzurwa;c.Kuraho gusohoka kuruhande rwinkingi, niba igitutu kitagabanutse, inkingi irahagaritswe.Umuti: Niba ari inzitizi yumunyu, kwoza 95% kugeza igihe igitutu gisanzwe.Niba inzitizi iterwa nibintu bimwe na bimwe byabitswe cyane, umuvuduko ukabije kuruta icyiciro cya mobile kigomba gukoreshwa kugirango wihute ugana umuvuduko usanzwe.Niba umuvuduko muremure wogusukura utagabanutse ukurikije uburyo bwavuzwe haruguru, kwinjira no gusohoka kwinkingi birashobora gufatwa nkaho bihujwe nigikoresho kinyuranyo, kandi inkingi irashobora guhanagurwa nicyiciro kigendanwa.Muri iki gihe, niba inkingi yumuvuduko itaragabanutse, inkingi yinjira mumashanyarazi isahani irashobora gusimburwa gusa, ariko iyo ibikorwa bitameze neza, biroroshye kuganisha kugabanuka kwingaruka zinkingi, gerageza rero gukoresha bike.Kubibazo bitoroshye, gusimbuza inkingi birashobora gusuzumwa.
(2) Igenamigambi ridakwiye: Igipimo gikwiye gishobora gusubirwamo.
.Niba bishoboka, umusemburo wo hasi wijimye urashobora gusimburwa cyangwa kongera gushyirwaho no gutegurwa.
(4) Umuvuduko wa sisitemu zero drift: hindura zeru ya sensor urwego rwamazi.
2, igitutu ni gito cyane (1) mubisanzwe biterwa na sisitemu.Icyo gukora: Shakisha buri gihuza, cyane cyane intera kumpande zombi zinkingi, hanyuma ukomere ahantu hasohotse.Kuraho inyandiko hanyuma ukomere cyangwa umurongo wa firime PTFE n'imbaraga zikwiye.
(2) Gazi yinjira muri pompe, ariko ubusanzwe igitutu ntigihinduka muriki gihe, hejuru kandi hasi.Ikirenzeho, pompe ntishobora gukuramo amazi.Uburyo bwo kuvura: fungura valve isukura hanyuma usukure ku kigero cya 3 ~ 5ml / min.Niba atari byo, ibyuka byo mu kirere byifuzaga kuri valve ikoresheje umuyoboro wabigenewe.
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(4) Indangantego yerekana ntabwo ifunze: valve yerekana ifunze nyuma yo kwihuta.Ubusanzwe iramanuka igera kuri 0.1.~ 0.2mL / min nyuma yo gufunga indangagaciro.
Incamake:
Muri iyi nyandiko, hasesenguwe gusa ibibazo bisanzwe muri chromatografiya.Nibyo, mubikorwa byacu bifatika, tuzahura nibindi bibazo byinshi.Mugukemura amakosa, dukwiye gukurikiza amahame akurikira: gusa duhindure ikintu kimwe icyarimwe kugirango tumenye isano iri hagati yibitekerezo nibibazo;Mubisanzwe, mugihe dusimbuye ibice kugirango dukemure ibibazo, dukwiye kwitondera gusubiza ibice bitavunitse byasubijwe inyuma kugirango birinde imyanda;Gushiraho ingeso nziza ni urufunguzo rwo gutsinda amakosa.Mu gusoza, iyo ukoresheje HPLC, ni ngombwa kwitondera icyitegererezo cyo kwitegura no gukora neza no gufata neza ibikoresho.
Igihe cyo kohereza: Nzeri-18-2023