Recombinant protein antigens ikunze kugira epitopes zitandukanye, zimwe murizo epitopa zikurikirana naho izindi ni epitopi yubatswe.Antibodiyite za polyclone zabonetse mugukingira inyamaswa hamwe na antigene zivanze ni uruvange rwa antibodi zihariye epitopi yihariye kandi irashobora gukoreshwa muri rusange kugirango tumenye imiterere karemano cyangwa poroteyine zigamije.Inyungu yo gukoresha poroteyine zitandukanijwe nka immunogène ni uko poroteyine zanduye zikunda kuba immunogene kandi zishobora gutera ubudahangarwa bw'umubiri ku nyamaswa.
Sisitemu yo kwerekana imvugo ya Escherichia coli isanzwe ihitamo intego za antigenic kuko niyo sisitemu ihenze cyane mugihe cyamafaranga.Kugirango tunoze ibishoboka byerekana intego ya poroteyine igamije kandi byorohewe no kwezwa, rimwe na rimwe gusa agace gato ka poroteyine yagenewe, nka domaine yihariye, iragaragazwa.
Imiterere-yuburyo butatu bwa poroteyine
Imiterere yihariye ya poroteyine
Niba intego yo gutegura antibody ari iyo kumenya gusa wb, ni ubukungu kandi byihuse gukoresha peptide ntoya ya peptide nka antigen, ariko harikibazo cyo gukingira indwara nkeya cyangwa kutavuka bitewe no guhitamo bidakwiye igice cya peptide.Kubera ko gutegura antibody bisaba igihe kirekire, hatoranijwe ibice bibiri cyangwa bitatu bitandukanye bya peptide kugirango bategure antibodies bakoresheje antigen polypeptide kugirango barebe ko ubushakashatsi bwatsinze.
Isuku ya polypeptide antigen yo gukingirwa irasabwa kuba hejuru ya 80%.Nubwo isuku ihanitse ishobora kubona antibodiyite zifite umwihariko, mubikorwa, inyamanswa zihora zitanga umubare munini wa antibodiyite zidasanzwe, bityo zigahisha inyungu ziterwa na antigen.
Byongeye kandi, gutegura antibodies ziva muri peptide nto bigomba guhuzwa na antigen ikwirakwiza kugirango yongere ubudahangarwa bwayo.Ibintu bibiri bisanzwe bitwara antigenic ni KLH na BSA.
Igihe cyo kohereza: Werurwe-23-2023