Ibibazo nibisubizo bya synthesis ndende

Mubushakashatsi bwibinyabuzima, polypeptide ifite urutonde rurerure ikoreshwa.Kuri peptide hamwe na aside amine zirenga 60 zikurikiranye, imvugo ya gene na SDS-PAGE ikoreshwa muburyo bwo kubibona.Nyamara, ubu buryo bufata igihe kirekire kandi ingaruka zanyuma zo gutandukanya ibicuruzwa ntabwo ari nziza.

Ibibazo nibisubizo bya synthesis ndende

Muri synthesis ya peptide ndende, duhora duhura nikibazo, ni ukuvuga ko inzitizi zikomeye ziterwa na kondegene ziyongera hamwe no kwiyongera kwurukurikirane muri synthesis, kandi igihe cyo kubyitwaramo kigomba guhinduka kugirango reaction irangire.Nyamara, igihe kinini cyo kubyitwaramo, niko ingaruka nyinshi zibyara umusaruro, kandi igice cyintego peptide kirakorwa.Ibisigisigi - iminyururu ya peptide ibuze ni umwanda wingenzi ukorwa muri synthesis ndende.Kubwibyo, muri synthesis ya peptide ndende, ikibazo cyingenzi tugomba gutsinda ni ugushakisha uburyo bwiza bwo kwitwara neza hamwe nuburyo bwo kubyitwaramo, kugirango tumenye aside amine acide yuzuye kandi yuzuye.Mubyongeyeho, gabanya igihe cyo kubyitwaramo, kuko igihe kinini cyo kubyitwaramo, niko impande zidashobora kugenzurwa, niko bigenda byiyongera kubicuruzwa.Kubwibyo, ingingo eshatu zikurikira zikubiye muri make:

Synthesis ya Microwave irashobora gukoreshwa: Kuri aminide acide imwe nimwe ihura mugikorwa cya synthesis itoroshye guhuzwa, synthesis ya microwave irashobora gukoreshwa.Ubu buryo bufite ibisubizo bitangaje, kandi bugabanya cyane igihe cyo kubyitwaramo, kandi bugabanya ishingwa ryibintu bibiri byingenzi by-ibicuruzwa.

Uburyo bwo guhuza ibice burashobora gukoreshwa.Ubu buryo bushobora kandi gukemura ibibazo byinshi muri synthesis.

Synthesis ya Acylhydrazide irashobora gukoreshwa: acylhydrazide synthesis ya peptide nuburyo bwo guhuza icyiciro gikomeye cya N-terminal Cys peptide na C-terminal polypeptide hydrazide imiti ihitamo imiti hagati yo gushiraho amide kugirango igere kuburyo bwa peptide.Ukurikije umwanya wa Cys mumurongo wa peptide, ubu buryo bugabanya urunigi rwa peptide yose uko yakabaye kandi ikomatanya.Ubwanyuma, intego peptide iboneka binyuze mumazi-fonction reaction.Ubu buryo ntibugabanya cyane igihe cyo guhuza peptide ndende, ariko kandi byongera cyane ubuziranenge bwibicuruzwa byanyuma.

Kweza peptide ndende

Umwihariko wa peptide ndende byanze bikunze biganisha kubintu bigoye bya peptide.Kubwibyo, biragoye kandi kweza peptide ndende na HPLC.amyloide yuruhererekane rwo kweza polypeptide, ikuramo uburambe bwinshi kandi ikoreshwa neza mugusukura peptide ndende.Mugukoresha ibikoresho bishya, kuvanga sisitemu nyinshi zo kweza, gutandukana inshuro nyinshi nubundi buryo bwuburambe, igipimo cyo gutsinda cya peptide ndende cyatejwe imbere cyane.