Thymus uburyo bushya bugizwe na 28 aminide acide synthique polypeptide yumubiri.Uburyo bwa Thymus ni ubwoko bwa uct imiti mishya, ikoreshwa kenshi mubarwayi b'ibibyimba, irashobora kongera ubudahangarwa.
Uburyo bwibikorwa
Uburyo nyabwo bwa thymalfasine mu kuvura indwara ya hepatite B idakira ntabwo bwigeze bwigwa neza, ariko ubushakashatsi butandukanye bwerekanye ko thymalfasine ifite ubudahangarwa bukomeye bw’umubiri.Byakozwe cyane cyane mugutezimbere imikorere ya T selile, kandi ikanyura muri leta zirimo umusaruro wa T selile, kugabana no gutunganya, na apoptose.Mu kongera umubare wuzuye wa lymphoide, intrahepatic naturel yica, CD3 na CD4, thymalfasin irashobora kugira uruhare mukudakingira.Pleural fasin irashobora guteza imbere gukura no kugabana ingirabuzimafatizo za DC, bityo bigatera gukura no kugabana kwa selile.Thymalfasin irashobora gukora mitogen cyangwa antigen, ikongera umusaruro wa cytokine zitandukanye nka interferons muntu interleukin IL2 na IL3, ikongera imvugo ya reseptor ya interleukin-2, kandi bigatuma CD4T itandukanya THLS, ifite ingaruka zikomeye za virusi.Mu kongera uruhare rw'uturemangingo P, irashobora guteza imbere umusaruro wa lymphocytes muri vivo, igahindura igipimo cya sisitemu ya P selile hamwe na U selile itandukanye, hamwe no gukora antibodiyite, bigatera umusaruro wa lymphokines na virusi ya virusi, bikongera kumira imikorere, no kunoza imikorere yubudahangarwa bw'umubiri ku barwayi ba kanseri.Irashobora kandi gukuraho bagiteri, bagiteri na virusi mu mubiri.Igitabo cyuburyo bwa Thymus kuri sisitemu ya nervice hamwe na endotelium ikikije, kuzamuka kugirango uhindure uruhare rwa hippocampal neuron synaptic kwanduza.Uburyo bwa Thymus uburyo bushya bwo kwanduza synaptic na sisitemu ya nervice, burashobora gukoreshwa mukurinda abarwayi ba kanseri batewe no kuvura kanseri nka chimiotherapie na neurotoxicity.Pleuraxin igira ingaruka zoguhindura gene, irashobora gutera genase ya casase cascade, kongera genes za cytokine, gen igenga immunite.Kugenzura-thymalfasine kuri gen-reseptor ya IL yerekana ko thymalfasine itera antigen itanga selile.Ati: “Byongeye kandi, ingaruka za thymalfasine ku mikurire y'ingirabuzimafatizo zigaragarira mu kwiyongera kw'imiterere ikura ry'ingirabuzimafatizo hamwe na poroteyine ya hematopoietic.”
Urutonde rushya rwa thymectomy
Inzira yo kwitegura
1. Uburyo bwo kuvoma biohimiki Uburyo bwa Thymosine butegura (imvange ya peptide) ahanini buboneka mubice byinyamanswa, bityo ibikorwa biratandukanye cyane.Kuberako isanzwe irimo proteine yinyamanswa, hari amahirwe menshi yo kwerekana ibimenyetso bya allergique.Uburyo bwa biohimiki bwabonetse bwa thymus buva muri embryonic thymus yumuntu ni shyashya, hamwe numusaruro muke cyane ariko ni byiza cyane.Urebye ibikoresho bike, igiciro kinini, umusaruro muke, hamwe nuburyo bwo kugura ibintu., ingorane nini zo kubyara umusaruro, ibyiringiro byiterambere ntabwo ari byiza.
2. Synthesis ya chimique Ubucuruzi buboneka thymusfasin ikorwa cyane cyane nka SPSS, ikorwa kandi igasukurwa nubuhanga bukwiye.Hashingiwe ku bisigarira, Fmoc yakoreshejwe mu gushiraho icyiciro gikomeye, kandi amoko 28 ya aside amine yakozwe hakurikijwe thymalfasine nayo.N-terminal aminide acide yashizwemo na anhydride ya acetike, kandi TFA yakoreshejwe nka reagent ikomeye yo kumena kugirango ibone ibicuruzwa bitoroshye.Igicuruzwa cyibanze cyatandukanijwe na HPLC kugirango kibone ibicuruzwa bishya bya thymus, hanyuma bisukurwa neza kugirango ubone uburyo bushya bwa thymus ibikoresho fatizo.
Hariho ubwoko butatu bwa bagiteri yakozwe na genetique
1) Ingirabuzimafatizo nyinshi zashyizwe mububiko kugirango zibone ingirabuzimafatizo, hanyuma zigaragazwa muburyo bwimibiri.Byavuzwe ko thymmethod novel gen-imirongo myinshi igizwe na tekinoroji ya annealing na PCR tekinike hanyuma ikorerwa muri Escherichia coli..Kurugero, hashingiwe ku kimenyetso cya peptide gene muri plasmid, imvugo plasmid yarubatswe, ihinduka pasitori ya Pichia na electroexcitation, hanyuma proteine ya fusion iterwa na methanol, hanyuma uburyo bushya bwa thymus bwubatswe no gusya enzyme nubundi buryo .
Igihe cyo kohereza: Nzeri-08-2023