Guhindura methylation

Methylation-yahinduwe peptide, izwi kandi nka peptide izwi na methylation, ni imitunganyirize ya poroteyine nyuma yubusobanuro (PTMs) kandi igira uruhare runini mubikorwa byubuzima hafi ya byose.Poroteyine ziterwa na methyltransferase kugirango yimure amatsinda ya hydroxyl mu bisigara byihariye bya aside amine kugirango bihuze.Methylation ni uburyo bwo guhindura ibintu buterwa na demethylase.

Ubushakashatsi bwerekanye ko aside methylated / demethylated amino acide isanzwe ari lysine (Lys) na arginine (Arg).Ubushakashatsi bwerekanye ko methylation ya histone lysine ifite ibikorwa bitandukanye byibinyabuzima nko kubungabunga ingirabuzimafatizo no kugabana, X chromosome idakora, kugenzura inyandiko-mvugo no gusubiza ibyangiritse kuri ADN.“, Ubusanzwe bigira ingaruka kuri chromatine kandi bikagabanya imvugo ya gene.”Methylation ya Histone arginine igira uruhare runini mugutunganya iyandikwa rya gene kandi irashobora kugira ingaruka kumikorere itandukanye ya selile, harimo gusana ADN, kwanduza ibimenyetso, iterambere rya selile, na kanseri.Kubwibyo, Guopeptide Biology yateje imbere byumwihariko tekinoroji ya methyl decorative peptide, ihindurwa nabahanga nyuma yo guhindura poroteyine (PTMS) kugirango ifashe mubushakashatsi.

Guhindura Methylation (Me1, Me2, Me3)

Ubwiza buhanitse Fmoc-Lys (Me, Boc) -OH, Fmoc-Lys (Me2) -OH, Fmoc-Lys (Me3) -OH.HCL, Fmoc-Arg (Me, Pbf) -OH, Fmoc-Arg (Me) 2-OH.HCl. byahanaguwe na HPLC.Ibyerekezo rusange bijyanye, chromatogramu ya HPLC na COA zitangwa kubicuruzwa byarangiye.