Nigute ihame ryo guhuza peptide muri peptide yihariye synthesis？

Ku buso, gushiraho imiyoboro ya peptide, itanga dipeptide, ni inzira yoroshye yimiti.Ibi bivuze ko ibice bibiri bya aside amine bihujwe na peptide, peptide ya amide, mugihe idafite umwuma.

Peptide yububiko ni ugukora aside amine mugihe cyoroheje..“Niba ibice bya karubisi (A) bidakingiwe, imiterere ya peptide ntishobora kugenzurwa.”Ibicuruzwa nkumurongo na peptide peptide birashobora kuvangwa nibintu bigenewe AB.Kubwibyo, amatsinda yose akora atagize uruhare mugushinga peptide agomba kurindwa muburyo bwigihe gito mugihe cya peptide.

Noneho, peptide synthesis - gushiraho buri peptide ihuza - ikubiyemo intambwe eshatu zo guteranya.

Intambwe yambere nugutegura aside amine ikenera kurindwa, kandi imiterere ya zwitterionic ya acide amino ntikibaho.

Intambwe ya kabiri ni intambwe ebyiri zifatika zo gukora peptide, aho itsinda rya carboxyl ya acide ya N irinzwe na N yabanje gukorerwa hagati ikora hanyuma noneho peptide ikorwa.Iyi reaction ihujwe irashobora kubaho nkintambwe imwe reaction cyangwa nkibisubizo bibiri bikurikiranye.

Intambwe ya gatatu ni ugukuraho guhitamo cyangwa gukuraho burundu ishingiro ryikingira.Nubwo kuvanaho byose bishobora kubaho nyuma yiminyururu ya peptide yose imaze guteranyirizwa hamwe, birasabwa kandi kuvanaho amatsinda arinda kugirango bikomeze peptide.

Kuberako acide 10 amine (Ser, Thr, Tyr, Asp, Glu, Lys, Arg, His, Sec na Cys) ikubiyemo amatsinda yimikorere yuruhande, bisaba kurindwa guhitamo, bigatuma synthesis ya peptide igorana.Ibirindiro byigihe gito nigice gihoraho bigomba gutandukanywa kubera ibisabwa bitandukanye kugirango uhitemo.Amatsinda yo kurinda by'agateganyo akoreshwa mu ntambwe ikurikira kugira ngo agaragaze uburinzi bw'agateganyo bwa aside amine cyangwa carboxyl ikora.Amatsinda arinda igice cyahoraho akurwaho atabangamiye imiyoboro ya peptide isanzwe cyangwa iminyururu ya aside amine, rimwe na rimwe mugihe cyo gukora.

Ati: "Byiza, ibikorwa bya carboxyl hamwe no gushinga peptide ya peptide (reaction reaction) bigomba kwihuta, hatabayeho amoko cyangwa ibicuruzwa biva mu mahanga, kandi bigomba gukoreshwa kugirango habeho umusaruro mwinshi."Kubwamahirwe make, ntanumwe muburyo bwo guhuza imiti wujuje ibi bisabwa, kandi bike bikwiranye na synthesis ifatika.

Mugihe cya peptide synthesis, amatsinda yibikorwa agira uruhare mubikorwa bitandukanye mubisanzwe ahujwe na santere yintoki, glycine niyo yonyine idasanzwe, kandi harikibazo gishobora kuzunguruka.

Intambwe yanyuma muri peptide synthesis cycle ni ugukuraho amatsinda yose arinda.Gukuraho guhitamo amatsinda arinda ni ngombwa mugukwirakwiza urunigi rwa peptide hiyongereyeho ibisabwa kugirango ukureho burundu uburinzi muri synthesis ya dipeptide.Ingamba zubukorikori zigomba gutegurwa neza.Ukurikije guhitamo ingamba, N irashobora guhitamo gukuraho α-amino cyangwa carboxyl irinda amatsinda.Ijambo "stratégie" ryerekeza ku ruhererekane rw'imikorere ya acide ya amine.Muri rusange, hari itandukaniro hagati ya synthesis gahoro gahoro hamwe nuduce duto.Peptide synthesis (izwi kandi nka "synthesis isanzwe") ibaho mubisubizo.Kenshi na kenshi, kwagura buhoro buhoro urunigi rwa peptide birashobora guhuzwa gusa ukoresheje urunigi rwa peptide kugirango uhuze ibice bigufi.Kugirango ushushanye peptide ndende, molekile yintego igomba kugabanywamo ibice bikwiye kandi ikemeza ko ishobora kugabanya urugero rwo gutandukana kuri C terminus.Nyuma yuko ibice byihariye byegeranijwe buhoro buhoro, intego yo guhuza izahuzwa.Ingamba zo guhuza peptide zirimo guhitamo igice cyiza kandi gikwiye cyo kurinda, kandi ingamba zo guhuza peptide zirimo guhitamo uburyo bukwiye bwo guhuza ibice bikingira hamwe nuburyo bwiza bwo guhuza ibice.