Ukurikije uburyo bwo guhuza aside amine nisukari, peptide yisukari irashobora kugabanywamo ibyiciro bine: O glycosylation, C a N glycosylation, isukari yikime na GPI (glycophosphatidlyinositol).
1 ishoboye guhuza urunigi rwa glycan igomba kuba iri muri AsN-X-Ser / Thr (X! = P) muri motif yakozwe nibisigisigi.Isukari ni N-acetylglucosamine.
N-glycosylation yahinduye imiterere ya glycopeptide
2. Imiterere ya O-glycosylation iroroshye kuruta N-glycosylation.Iyi glycopeptide muri rusange ni ngufi kuruta glycan, ariko ifite ubwoko bwinshi kuruta N-glycosylation.Ser na Thr birashobora kuba glycosylated mumurongo wa peptide.Byongeye kandi, hari glycopeptide irimbishijwe na tirozine, hydroxyl, na hydroxyproline glycosylation.Umwanya uhuza ni hydroxyl ogisijeni atom kumurongo wuruhande rwibisigisigi.Isukari ihujwe ni galactose cyangwa N-acetylgalactosamine (Gal & GalNAc) cyangwa glucose / glucosamine (Glc / GlcNAc), mannose / mannosamine (Umuntu / ManNAc), nibindi.
O-glycosylation ihindura imiterere
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine / acetylglucosamine)
Glycosylation ya N-acetylglucosamine imwe (GlcNAc) ihuza poroteyine O-GlcNAc na hydroxyl ogisijeni atom ya serine cyangwa ibisigazwa bya proteine.O-GlcNA glycosylation ni O-GlcNAc monosaccharide imitako idafite glycan;Kimwe na peptide fosifora, O-GlcNAc glycosylation ya glycopeptides nayo ni inzira yo gushushanya poroteyine.Imitako idasanzwe ya O-GlcNAc irashobora gutera indwara zitandukanye nka diyabete, indwara zifata umutima, ibibyimba, indwara ya Alzheimer nibindi.
Ingingo ya Glycosylation ya glycopeptides
Imiterere yibanze ya polypeptide nu munyururu wisukari ihujwe nuruhererekane rwa poroteyine na covalent bonds, naho ibibanza bihuza iminyururu yisukari byitwa glycosylation site.Kubera ko nta shusho yo gukurikiza biosynthesis ya glycopeptide iminyururu yisukari, iminyururu itandukanye yisukari izahuzwa kumurongo umwe wa glycosylation, biganisha kubyo bita microscopique inhomogeneity.
Glycosylation ya glycopeptides
1. Ingaruka za glycopeptide glycosylation kuri therapy-efficacy ya proteine zo kuvura
Kubijyanye na proteine zo kuvura-kuvura, glycosylation nayo igira ingaruka ku gice cya kabiri cyubuzima no kwibasira imiti ya poroteyine muri vivo
2. Gukuramo glycopeptide glycosylation na proteyine
Ubushakashatsi bwerekanye ko urunigi rw'isukari hejuru ya poroteyine rushobora kuzamura molekile ya poroteyine
3. Glycopeptide glycosylation na immunogenicite ya protein
Ku ruhande rumwe, iminyururu yisukari hejuru ya poroteyine irashobora gutera ubudahangarwa bw'umubiri.Ku rundi ruhande, urunigi rw'isukari rushobora gupfuka hejuru ya poroteyine no kugabanya ubudahangarwa bwarwo
4. Glycopeptide glycosylation yongerera poroteyine
Glycosylation irashobora kongera intungamubiri za poroteyine mubihe bitandukanye byo gutandukana (nka denaturants, ubushyuhe, nibindi) kandi ikirinda kwegeranya poroteyine.Muri icyo gihe, urunigi rw'isukari hejuru ya poroteyine rushobora kandi gutwikira ingingo zimwe na zimwe zangirika za poroteyine za poroteyine, bityo bikongera imbaraga za poroteyine kuri poroteyine.
5. Glycopeptide glycosylation igira ingaruka kubikorwa byibinyabuzima bya molekile ya poroteyine
Guhindura protein glycosylation birashobora kandi gutuma molekile ya poroteyine ikora ibikorwa bishya byibinyabuzima
Igihe cyo kohereza: Kanama-03-2023