Igishushanyo mbonera nigisubizo cya polypeptide peptide

I. Incamake
Peptide ni macromolecules idasanzwe kuburyo urutonde rwarwo rudasanzwe mumiterere yimiti niyumubiri.Peptide zimwe ziragoye guhuza, mugihe izindi ziroroshye guhuza ariko biragoye kubisukura.Ikibazo gifatika nuko peptide nyinshi zishonga gake mubisubizo byamazi, kubwibyo rero mu kwezwa kwacu, igice gikwiranye na peptide ya hydrophobique kigomba gushonga mumashanyarazi adafite amazi, Kubwibyo, iyi mashanyarazi cyangwa buffers birashoboka ko bidahuye cyane nikoreshwa yuburyo bwibigeragezo bwibinyabuzima, kuburyo abatekinisiye babujijwe rwose gukoresha peptide kubyo bagamije, kuburyo ibikurikira aribintu byinshi bigize igishushanyo mbonera cya peptide kubashakashatsi.

Igishushanyo mbonera nigisubizo cya polypeptide peptide
Icya kabiri, guhitamo neza peptide ikora neza
1. Uburebure bwuzuye bwurwego rwateganijwe
Peptide yibisigazwa bitarenze 15 biroroshye kubibona kuko ubunini bwa peptide bwiyongera kandi ubuziranenge bwibicuruzwa bikagabanuka.Nkuko uburebure bwuzuye bwurunigi rwa peptide bwiyongera burenze ibisigisigi 20, ubwinshi bwibicuruzwa nibintu byingenzi.Mubigeragezo byinshi, biroroshye kubona ingaruka zitunguranye mugabanya umubare usigaye munsi ya 20.
2. Kugabanya umubare wibisigazwa bya hydrophobi
Peptide yiganjemo ibisigazwa bya hydrophobique, cyane cyane mukarere 7-12 ibisigazwa bya C-terminus, mubisanzwe bitera ingorane.Ibi bigaragara nkikomatanya ridahagije neza kuberako urupapuro rwa B rwabonetse muri synthesis.Ati: "Mu bihe nk'ibi, birashobora kuba byiza guhindura ibisigisigi birenga bibiri byiza kandi bibi, cyangwa gushyira Gly cyangwa Pro muri peptide kugirango ufungure peptide."
3. Kugabanya ibisigazwa "bigoye"
“Hariho Cys, Met, Arg, na Gerageza ibisigara muri rusange bitoroshye guhuzwa.”Ser izakoreshwa muburyo budasanzwe bwa Cys.
Igishushanyo mbonera nigisubizo cya polypeptide peptide

Icya gatatu, kunoza amahitamo meza yo gushonga mumazi
1. Hindura ijambo N cyangwa C.
Ugereranije na peptide acide (ni ukuvuga, yishyuwe nabi kuri pH 7), acetylation (N-terminus acetylation, C terminus ihora ikomeza itsinda rya karubisi yubusa) irasabwa cyane cyane kongera amafaranga mabi.Nyamara, kuri peptide yibanze (ni ukuvuga, yishyuwe neza kuri pH 7), amination (amine yubusa kuri N-terminus na amination kuri C-terminus) birasabwa cyane cyane kongera amafaranga meza.

2. Gabanya cyane cyangwa wongere urutonde

Bimwe mubikurikiranye birimo umubare munini wa hydrophobique amino acide, nka Trp, Phe, Val, Ile, Leu, Met, Tyr na Ala, nibindi. Iyo ibyo bisigazwa bya hydrophobi birenze 50%, mubisanzwe ntabwo byoroshye gushonga.Birashobora kuba ingirakamaro kurambura urukurikirane kugirango turusheho kongera inkingi nziza kandi mbi ya peptide.Ihitamo rya kabiri ni ukugabanya ingano yumunyururu wa peptide kugirango wongere inkingi nziza nibibi muguhindura ibisigazwa bya hydrophobi.Nukomera cyane impande nziza kandi mbi zurunigi rwa peptide, niko bishoboka cyane gufata amazi.
3. Shyiramo ibisigazwa byamazi
Ku munyururu wa peptide, guhuza aside amine nziza kandi mbi birashobora kunoza amazi.Isosiyete yacu irasaba N-terminus cyangwa C-terminus ya peptide acide guhuzwa na Glu-Glu.N cyangwa C terminus ya peptide yibanze yatanzwe hanyuma Lys-Lys.Niba itsinda ryishyuzwa ridashobora gushyirwa, Ser-Gly-Ser irashobora kandi gushyirwa muri N cyangwa C.Nyamara, ubu buryo ntibukora mugihe impande zumunyururu wa peptide zidashobora guhinduka.