Ibisobanuro
Dipeptide-6igaragara nkifu yera yera yijimye kandi irashonga mumazi.Guhuza neza nuruhu.Biroroshye kubyakira.Ingaruka nziza yo kurwanya gusaza, ingaruka zo kurinda uruhu.
Ibisobanuro
Kugaragara: Ifu yera-yera-ifu
Isuku (HPLC):≥98.0%
Umwanda umwe:≤2.0%
Ibirimo Acetate (HPLC): 5.0%~12.0%
Ibirimo Amazi (Karl Fischer):≤10.0%
Peptide Ibirimo:≥80.0%
Gupakira no kohereza: Ubushyuhe buke, gupakira vacuum, neza kuri mg nkuko bisabwa.
Nigute ushobora gutumiza?
1. Contact us directly by phone or email: +86-13735575465, sales1@gotopbio.com.
2. Tegeka kumurongo.Nyamuneka wuzuze urupapuro rwabigenewe.
3. Tanga izina rya peptide, CAS Oya cyangwa urutonde, ubuziranenge no guhindura niba bikenewe, ubwinshi, nibindi tuzatanga cote mumasaha 2.
4. Tegeka guhinduka ukoresheje amasezerano yo kugurisha yasinywe byemewe na NDA (amasezerano yo kutamenyekanisha) cyangwa amasezerano yibanga.
5. Tuzakomeza kuvugurura iterambere ryateganijwe mugihe.
6. Gutanga peptide na DHL, Fedex cyangwa abandi, na HPLC, MS, COA bizatangwa hamwe n'imizigo.
7. Politiki yo gusubizwa izakurikizwa niba hari itandukaniro ryubwiza cyangwa serivisi.
8. Serivisi nyuma yo kugurisha: Niba abakiriya bacu bafite ikibazo kijyanye na peptide mugihe cyibigeragezo, nyamuneka twandikire natwe tuzagusubiza mugihe gito.
Ibicuruzwa byose byikigo bikoreshwa gusa mubushakashatsi bwubumenyi, ni's bibujijwe gukoreshwa mu buryo butaziguye n'abantu bose ku mubiri w'umuntu.
Ibibazo
Ni izihe mbogamizi ku burebure bwa peptide?
Polypeptide ikomatanya nisosiyete yacu ni 6 ~ 50 acide aminide.Uburyo busanzwe bukomeye bwa synthesis isanzwe itanga peptide ya acide 6 kugeza kuri 50.
Nigute ushobora gusobanura impinga ya P + Na na P + K muri MALDI (MS)?
Impinga Na na K zikunze kugaragara muri MALDI, kandi sodium na potasiyumu biva mumazi adasanzwe.Ndetse n'amazi yatoboye kandi ya deionion arashobora kuba arimo urugero rwa sodium na potasiyumu ion zidashobora kuvaho burundu.Barashobora kandi ionize kandi bagahuza na carboxyl yubusa ya peptide mugihe cya spekrometrike.Kubera ko nta gahunda yo kweza ikuraho sodium na potasiyumu ion mu mazi, rimwe na rimwe byanze bikunze byanze bikunze impinga ya sodium na potasiyumu igaragara ku ikarita ya MALDI MS.
Nyamuneka sobanura ingamba zawe zo kwezwa
Peptide ikomatanya nisosiyete yacu yasukuwe nimyiteguro ihindagurika-icyiciro cya HPLC hamwe na TFA na pH 2 byongewe mubice byombi bigendanwa.Icyiciro A ni 0.1% TFA mumazi ya ultra-yera, naho icyiciro B ni 0.1% TFA muri ACN, pH 2. Icyitegererezo gishobora gushonga muburyo butaziguye, cyangwa kigashonga mugice gito cya B hanyuma kikavangwa nicyiciro A.Rimwe na rimwe, birashobora kuba nkenerwa gushonga peptide hydrophobique hamwe na solide ikomeye nka acide formic cyangwa acide acike, bitewe na peptide ikurikirana.Kuri pH 6.8, mubisanzwe biragoye gushonga no kweza peptide, kubwibyo rero muri rusange tubanza gushonga peptide hanyuma tugakoresha ibyiciro bibiri bigendanwa kugirango bikureho buhoro buhoro.Igisubizo cya buffer kuri pH 6.8 cyari 10 mM ametiyumu acetate mumazi meza cyane (icyiciro cya mobile A), ACN yera (icyiciro cya mobile B).Ibice bitandukanye byakusanyirijwe hamwe no kumenyekana na MADLI-TOF MS.Isuku yasesenguwe na HPLC ihindagurika.Hanyuma, peptide yintego yashizwemo lisofile, hanyuma peptide ya lyofilize ihuzwa mubibindi bito.
Nigute ukurikirana ibicuruzwa byawe?
Peptide zose za sintetike zasesenguwe na HPLC na MS, usibye MALDIMS.Kubera ko peptide ikozwe muburyo bukurikiranye muburyo butandukanye, tekinoroji ya HPLC-MS irashobora gukoreshwa mugusesengura izo mpinga za ionisation muri HPLC.Ibikoresho byacu bya tekiniki [MALDI-MS, HPLC- (ESI) MS (ion trap na tetrode array)] bitanga ibyiringiro byizewe kubisesengura.
Ese gukomera kwa peptide bifitanye isano nubwiza bwa peptide?
Peptide ya syntetique ntishonga neza, peptide ifite ikibazo, sibyo?
Igisubizo: Biragoye guhanura neza uburyo peptide ikemuka nigisubizo gikwiye.Ntabwo arukuri ko hariho ikibazo cya synthesis ya peptide niba bigoye gushonga.
Nigute ushobora kubika peptide mugisubizo?
Niba ugomba kubika peptide yawe mumazi, koresha buffer sterile kuri PH 5-6 hanyuma ubike kuri -20℃kwagura ubuzima bwa peptide yawe mugukemura.
Peptide imara igihe kingana iki mugisubizo?
Nibyiza kutabika peptide isigaye mugisubizo.Ubuzima bwa tekinike ya polypeptide mugisubizo ni buke cyane cyane abafite sisitemu, methionine, tryptophan, aside asparagic, aside glutamic, na aside N-terminal glutamic ikurikirana.Muri rusange, fata urugero rukenewe rwo gukoresha, ahasigaye gukonjesha byumye kubikwa igihe kirekire.